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GENETIC AND BIOLOGICAL CHARACTERISTICS OF METARHIZIUM ANISOPLIAE ISOLATES ISOLATED FROM INSECT PESTS OF AGROECOSYSTEMS OF UZBEKISTAN REGIONS

Metarhizium anisopliae, entomopathogenic fungi, Syrdarya region, Kashkadarya region, Colorado potato beetle, biological plant protection.

Authors

  • Холмурод AБДУВАЛИЕВ Студент бакалавриата, Ташкентский государственный технический университет имени Ислама Каримова, Uzbekistan

This study presents the results of a comprehensive investigation of isolates of entomopathogenic fungi of the genus Metarhizium
obtained from insect pests of agroecosystems in the Syrdarya and Kashkadarya regions of the Republic of Uzbekistan. In total, 155
isolates were recovered from insect bodies, including 95 isolates from the Colorado potato beetle (Leptinotarsa decemlineata Say.)
and 60 isolates from larvae of the greater wax moth (Galleria mellonella). Biological screening was conducted to assess
antagonistic activity against Fusarium oxysporum and Fusarium solani, as well as the effects of promising isolates on cucumber
(Cucumis sativus L.) growth. Based on biochemical evaluation and molecular identification, ten strains were selected and assigned
to Metarhizium anisopliae. The selected strains were shown to produce conidial suspensions with titers of at least 1.0 × 10⁶
conidia/mL. The obtained data confirm the potential of local M. anisopliae strains for the development of biopreparations adapted
to the conditions of irrigated agriculture in the Syrdarya and Kashkadarya regions.