DETERMINATION OF THE ANTICOAGULANT PROPERTIES OF THE POLYPHENOLIC COMPOUND PC 9 IN RAT BLOOD PLASMA UNDER IN VITRO CONDITIONS
This article analyzes the results of coagulation tests - activated partial thromboplastin time (QFTV), prothrombin time (PT), and fibrinogen levels - obtained during the evaluation of the polyphenolic compound PC-9 as a novel anticoagulant agent. The experiments were conducted on male white Wistar rats weighing 200–300 g. QFTV and PT tests were performed using the classical coagulation method, while the fibrinogen level was measured using the Von Clauss method. All studies were carried out in accordance with Good Laboratory Practice (GLP) and the requirements established by the European Union Directive 2010/63/EU and organizations such as the Institutional Animal Care and Use Committee (IACUC) in the United States. Differences in coagulation time indicators and fibrinogen concentrations were identified between the experimental and control groups, allowing for the assessment of the anticoagulant efficacy of the compound. The coagulation tests conducted on the PC-9 polyphenolic compound demonstrated significant anticoagulant properties. Further in vivo models and clinical trials are recommended. Based on the results of this experiment, the compound shows potential for use as an anticoagulant.
1. Iroz, C. B., Dahl, C., Cassimatis, I. R., Wescott, A., & Miller, E. S, prophylactic anticoagulation for preterm premature rupture of membranes: a decision analysis//American Journal of Obstetrics & Gynecology, Maternal-fetal Medicine. 2021 Vol. 3. Is. 3. P. 100311. DOI: https://doi.org/10.1016/j.ajogmf.2021.100311
2. Lv, J., Li, Z., & Zhang, L. two new flavonoids from Artemisia argyi with their anticoagulation activities//Natural Product Research. 2017. Vol.32. Is. 6. P. 632–639. DOI:https://doi.org/10.1080/14786419.2017.1332603
3. Steinbrecher, O., Šinkovec, H., Eischer, L., Kyrle, P. A., & Eichinger, S. D-dimer levels over time after anticoagulation and the association with recurrent venous thromboembolism//Thrombosis Research. 2021. Vol. 19. P. 160–164. DOI: https://doi.org/10.1016/j.thromres.2020.11.015
4. Montinari, M. R., Minelli, S., & De Caterina, R.eighty years of oral anticoagulation: Learning from history//Vascular Pharmacology. 2021. Vol 141. P. 106918. DOI: https://doi.org/10.1016/j.vph.2021.106918
5. Ragab, T. I., Amer, H., Mossa, A. H., Emam, M., Hasaballah, A., & Helmy, W. A. anticoagulation, fibrinolytic and the cytotoxic activities of sulfated hemicellulose extracted from rice straw and husk// Biocatalysis and Agricultural Biotechnology. 2018. Vol. 15. P. 86–91. DOI: https://doi.org/10.1016/j.bcab.2018.05.010
6. Ferreira, J. L., & Wipf, J. pharmacologic therapies in anticoagulation// Medical Clinics of North America. 2016.˜Vol. 100 Is. 4. P. 695–718. DOI: https://doi.org/10.1016/j.mcna.2016.03.007 7. Lefebvre, S., Hascoët, C., Damin-Pernik, M., Rannou, B., Benoît, E., & Lattard, V. monitoring of antivitamin K-dependent anticoagulation in rodents – Towards an evolution of the methodology to detect resistance in rodents// Pesticide Biochemistry and Physiology. 2017. Vol. 138. P. 29–36. DOI: https://doi.org/10.1016/j.pestbp.2017.02.003
8. Gao, L., Wang, F., Chen, Y., Li, F., Han, B., & Liu, D. the antithrombotic activity of natural and synthetic coumarins// Fitoterapia. 2021. Vol. 154. P. 104947. DOI: https://doi.org/10.1016/j.fitote.2021.104947 9. Deng, X., Lei, H., Ren, Y., Ai, J., Li, Y., Liang, S., Chen, L., & Liao, M. a novel strategy for active compound efficacy status identification in multi-tropism Chinese herbal medicine (Scutellaria baicalensis Georgi) based on multi-indexes spectrumeffect gray correlation analysis//Journal of Ethnopharmacology. 2023. Vol. 300. P. 115677. DOI: https://doi.org/10.1016/j.jep.2022.115677
10. Cartwright, B., & Mundell, N. anticoagulation for cardiopulmonary bypass: part one//BJA Education. 2023. Vol. 23. Is. 3. P. 110–116. DOI: https://doi.org/10.1016/j.bjae.2022.12.003 11. Wang, J., Li, J., & Zhong, L. current status and prospect of anti-amyloid fibril therapy in AL amyloidosis//Blood Reviews. 2024. P. 101207. DOI:https://doi.org/10.1016/j.blre.2024.101207
Copyright (c) 2025 «ACTA NUUz»
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
.jpg)
1.png)
