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SPECTROPHOTOMETRIC DETERMINATION OF THE MOLAR ACTIVITY OF A RADIOPHARMACEUTICAL BASED ON PHOSPHORUS-32 RADIONUCLIDE

phosphorus-32, vanadium–molybdenum reagent, spectrophotometry, ammonium vanadate, ammonium molybdate, potassium dihydrogen phosphate, molar activity.

Authors

  • Lazizbek HAJIEV Falsafa doktori, O‘zR FA Yadro fizikasi instituti “Radiopreparat” davlat muassasasi yetakchi muhandisi,, Uzbekistan
  • Ilxom SADIKOV Texnika fanlari doktori, professor, akademik, O‘zR FA Yadro fizikasi instituti direktori, Uzbekistan
  • Аyubxan АBDUKAYUMOV Texnika fanlari doktori, O‘zR FA Yadro fizikasi instituti “Radiopreparat” davlat muassasai direktori, Uzbekistan
  • Abror RIHSIEV Texnika fanlari doktori, O‘zR FA Yadro fizikasi instituti “Radiopreparat” davlat muassasai bosh texnologi, Uzbekistan
Vol. 3 No. 3.1 (2026): O'zMU xabarlari 3.1\2026 Tabiiy fanlar
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In this study, a simple and sensitive spectrophotometric method was developed to determine the molar activity (Ci/mmol) of a
carrier-free phosphorus-32 labeled orthophosphoric acid radiopharmaceutical (H₃[³²P]PO₄). The method relies on the formation of
a yellow phospho– vanadium–molybdenum complex in nitric acid medium, showing a maximum absorbance at 410 nm. The
calibration equation was d = 0.07812·n + 0.00783 (n ×10 ⁸ mol), with R² = 0.9993. The⁻ limit of detection (LOD) was 0.17 µg and
the limit of quantification (LOQ) was 0.56 µg, with a linear working range of 0.31–4.96 µg of phosphorus. For the tested sample,
the molar activity was 8410 Ci/mmol and the deviation from the theoretical value was 1.06%.