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DETERMINATION OF RESISTANCE TO SALT STRESS IN MICROORGANISMS ISOLATED FROM SOILS OF THE NORTHERN REGIONS OF UZBEKISTAN

saline soil, Bacillus spp., halotolerant bacteria, salt tolerance, physiological and biochemical characteristics.

Authors

Microbial isolates were obtained from saline soil samples collected in the Aral Sea region and Khorezm Province and were
identified based on their morphological, physiological, and biochemical characteristics. In addition to classical microbiological
methods, modern approaches were also used for identification, which made it possible to accurately determine the taxonomic
position of the microorganisms. The halotolerance level of the isolated strains was studied based on their growth dynamics under
different NaCl concentrations. Salt tolerance testing was carried out within the range of 3–18% NaCl, which made it possible to
simulate conditions of extreme salinity. According to the results, Bacillus licheniformis exhibited the highest halotolerance,
maintaining growth even at 18% NaCl. Bacillus megaterium showed growth up to 15%, Bacillus atrophaeus and Bacillus
vallismortis up to 12%, while Bacillus pumilus and Pseudomonas koreensis retained growth capacity up to 10% NaCl.