THE EFFECT OF KAEMPFEROL FLAVONOID ON RESPIRATORY PROCESS OF RAT LIVER MITOCHONDRIA
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The effect of kaempferol flavonoid on oxidative phosphorylation process in mitochondria isolated from rat liver was studied. In our experiments, we used different concentrations of kaempferol flavonoid in the oxidation of succinate, a substrate dependent on FAD, and demonstrated the antioxidant properties of the studied flavonoid, partial division of the oxidative-phosphorylation process occurred under the influence of the studied substance.
1. Сaмaрцев В.Н., Дубинин М.В. Стимуляция пaльмитиновой кислотой окисления дофaминa в митохондриях печени в присутствии aтр и трет-бутилгидропероксидa // Биол. мембрaны. – 2016. – Т. 33(3).
2. Скулaчев В.П., Богaчев A.В., Кaспaринский Ф.О. Мембрaннaя биоэнергетикa // М.: МГУ, 2010. – 368 с.
3. Alberts B., Johnson A., Lewis J. et al. The mitochondrion. The Molecular Biology of the Cell // (4th ed.). – 2002. – P. 767768. 4. Andreas Kohler, Antoni Barrientos, Flavia Fontanesi, Martin Ott. The functional significance of mitochondrial respiratory chain supercomplexes. EMBO Rep 2023 Nov 6;24(11):e57092. doi: 10.15252/embr.202357092
5. Chance B., Williams G.R. The respiratory chain and oxidative phosphorilation // Adv.enzymol. – 1956. – V.17. – Р. 65-134.
6. Halliwell B., Gutteridge J. Free Radicals in Biology and Medicine // 3rd ed. Oxford University Press Inc. New York, NY1999.
7. Hélène Pelicano., Li Feng., Yan Zhou., William Plunkett., Michael J., Keating., Peng Huang. Inhibition of Mitochondrial Respiration // JOURNAL OF BIOLOGICAL CHEMISTRY. SEPTEMBER 2003. doi.org/10.1074/jbc.M301546200. P37832-37839,
8. Natalia Smolina, Joseph Bruton, Anna Kostareva, Thomas Sejersen. Assaying Mitochondrial Respiration as an Indicator of Cellular Metabolism and Fitness Methods // Mol Biol. 2017:1601:79-87. doi: 10.1007/978-1-4939-6960-9.
9. Richter C., Gogvadze V., Laffranchi R., Schlapbach R., Schweizer M., Suter M., Walter P., Yaffee M. Oxidants in mitochondria: from physiology to diseases // Biochim. Biophys. Acta. 1995; 1271: 67-74 10. Runyu Guo, Jinke Gu, Shuai Zong, Meng Wu, Maojun Yang. Structure and mechanism of mitochondrial electron transport chain. Biomed J. 2018 Feb;41(1):9-20. doi:
10.1016/j.bj.2017.12.001. Epub 2018 Mar 26 11. Saybasili H., Yuksel M., Haklar G., Yalcin A.S. Antioxid // Redox Signal. 2001. 3. 1099-1104. 12. Staniek K., Gille L., Kozlov A.V., Nohl H. Free Radic // Res. 2002. 36: 381-387.
13. Tanaka T., Saotome M., Katoh H. et. al. Glycogen synthase kiase-3β opens mitochondrial permeability transition pore through mitochondrial hexokinase II dissociation // J Physiol Sci. – 2018. –V. 68(6). –P. 865-871. 14. Toime L.J., Brand M.D. Uncoupling protein-3 lowers reactive oxygen species production in isolated mitochondria // Free Radic Biol Med. – 2010. – Vol. 49(4). – P. 606-611.
15. Yuriy Chaban, Egbert J Boekema, Natalya V Dudkina. Structures of mitochondrial oxidative phosphorylation supercomplexes and mechanisms for their stabilization // Biochim Biophys Acta. 2014 Apr;1837(4):418-26.
16. И. Рослый., Т. Демура Мы дышим… митохондриями печени // Фармвестник. – 2018. №9 (164). 17. Letts J.A., Sazanov L.A. Clarifying the supercomplex: the higher-order organization of the mitochondrial electron transport chain // Nat Struct Mol Biol. – 2017. – Vol. 24(10). – P. 800–808.
18. Mitchell P. Coupling of phosphorylation to electron and hydrogen transfer by a chemi-osmotic type of mechanism // Nature. – 1961. – Vol. 191. – P. 144–148.
19. Nolfi-Donegan D., Braganza A., Shiva S. Mitochondrial electron transport chain: Oxidative phosphorylation, oxidant production, and methods of measurement // Redox Biol. – 2020. – Vol. 37:101674.
20. Pham L., Arroum T., Wan J. et al. Regulation of mitochondrial oxidative phosphorylation through tight control of cytochrome c oxidase // Redox Biol. – 2024. – Vol. 78:103426.
21. Internet manbalari: 22. https://ru.wikipedia.org/wiki/Митохондрия 23. https://en.wikipedia.org/wiki/Chemiosmosis
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