SOG‘LOM KO‘CHATLAR OLISH UCHUN KUNGABOQAR (HELIANTHUS ANNUUS L.) NING IN VITRO URUG‘ STERILIZATSIYA PROTOKOLINI OPTIMIZATSIYA QILISH
##submission.downloads##
Ushbu tadqiqot kungaboqar (Helianthus annuus L.) urug‘larini in vitro sharoitida sterilizatsiya qilish protokollarini
optimallashtirishga, genetik transformatsiya va regeneratsiya bo‘yicha tadqiqotlar uchun ishonchli asos yaratishga qaratildi. Uch
xil kimyoviy sterilizator samaradorligi baholandi, bunda 70% etanol, 15% natriy gipoxlorit (NaOCl) Tween-20 bilan va 0,1%
kumush nitrat (AgNO₃) turli ekspozitsiya vaqtlarida. Natijalar shuni ko‘rsatdiki, 0,1% AgNO₃ eritmasida 2 daqiqa ishlov berish
eng yuqori unuvchanlik (90,6%)ni ta’minladi va ushbu usulning ustunligini namoyon etdi. Kumush nitrat asosida ishlab chiqilgan
O‘ZBEKISTON MILLIY
UNIVERSITETI
XABARLARI, 2025, [3/2/1]
ISSN 2181-7324
BIOLOGIYA
http://journals.nuu.uz
Natural sciences
O‘zMU xabarlari Вестник НУУз ACTA NUUz BIOLOGIYA 3/2/1 2025
- 96 -
optimallashtirilgan protokol sog‘lom, kontaminatsiyasiz ko‘chatlar olish uchun ishonchli va samarali usul bo‘lib, ayniqsa
O‘zbekiston kabi hududlarda qishloq xo‘jalik ekinlarining chidamliligini oshirish ustuvor vazifa bo‘lgan sharoitda dolzarb
ahamiyat kasb etadi.
1. Ali A., Muhammad A., Muhammad Z. (2018). Contamination problems in plant tissue culture and their management: A
review. Journal of Agricultural Sciences, 36(2), 1-10.
2. Barampuram S., Zhang Z.J. (2011). Recent advances in plant transformation. In: Plant Chromosome Engineering (pp. 1-35).
Springer.
3. Bhojwani S.S., Razdan M.K. (1996). Plant Tissue Culture: Theory and Practice. Elsevier.
4. Hemathilake H.M. Peiris S.E. (2021). Mini-Review on Methods of Explant Surface Sterilization for Efficient
Micropropagation. Proceedings of SLIIT International Conference on Advancements in Sciences & Humanities, 76-82.
5. Healy S.M., et al. (2021). Ethanol as a pre-treatment for explant surface sterilization in plant tissue culture. Plant Cell, Tissue
and Organ Culture, 147(1), 1-10.
6. Kumari, A., et al. (2017). Seed sterilization techniques in plant tissue culture: A review. International Journal of Current
Microbiology and Applied Sciences, 6(7), 453-460.
7. Mihaljevic, S., et al. (2013). Silver nitrate as an effective agent for in vitro surface-sterilization protocol of Taxus baccata L.
shoot explants. ResearchGate, 278728825.
8. Moreno-Vázquez, M. J., et al. (2014). Bacterial contamination of in vitro plant cultures: confounding effects on somaclonal
variation and detection of contamination in plant tissues. ResearchGate, 284942347.
9. Rout, G. R. (2011). In vitro plant regeneration and genetic transformation of sunflower. In: Plant Tissue Culture: An
Approach to Genetic Transformation (pp. 109-124). Springer.
10. Shahzad, A., & Anis, M. (2009). In vitro regeneration via somatic embryogenesis using cotyledon explants of sunflower.
Plant Biotech Reports, 3(3), 143-150.
11. Sujatha, M., & Prabakaran, A. J. (2003). Agrobacterium-mediated transformation of sunflower (Helianthus annuus L.):
factors affecting the efficiency of gene transfer. Plant Cell Reports, 21, 325–334.
12. Teixeira da Silva, J. A., et al. (2015). The Effect of Sodium Hypochlorite Solutions on the Viability and In Vitro Regeneration
Capacity of the Tissue. ResearchGate, 285883623.
Mulkiiyat (c) 2025 «O‘zMU XABARLARI»
Ushbu ish quyidagi litsenziya asosida ruxsatlangan Kreativ Commons Attribution-NonCommercial-ShareAlike 4.0 International litsenziyasi asosida bu ish ruxsatlangan..
.jpg)
.png)
