POLIETILENNI SAMARALI PARCHALASH UCHUN PETaza FERMENTINI ESCHERICHIA COLI BAKTERIYASIDA EXSPRESSIYALASH
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Plastik ifloslanish, birinchi navbatda, polietilen tereftalat (PET) plastmassalari bilan bog'liq bo'lib, jiddiy global ekologik inqirozga aylandi. Bakteriyaning Ideonella sakaiensis 201-F6 turi shtami PETni parchalash uchun eng samaralii yechim sifatida foydalaniladi. Biz ushbu maqolada, termostabil mutatsiyalarga ega bo’lgan FAST-PETaza fermentini klonlash va uni E.coli bakteriyasida ekspressiyalash, oqsilni nikel xelat xromotagrafiya usulida tozalab olish va ferment aktivligini aniqlash kabi tadqiqot natijalarini bayon etdik. Tadqiqot natijalari aktivlikga ega bo’lgan FAST-PETaza fermentini samarali tozalab olinganini ko’rsatdi.
Muhammad Latif NAZIROV, Ilxom XALILOV, & Fazliddin QOBILOV,. (2024). POLIETILENNI SAMARALI PARCHALASH UCHUN PETaza FERMENTINI ESCHERICHIA COLI BAKTERIYASIDA EXSPRESSIYALASH. «O‘zMU XABARLARI», 3(3.1), 81–85. https://doi.org/10.69617/nuuz.v3i3.1.1718
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2. Han X, Liu W, Huang JW, Ma J, Zheng Y, Ko TP, Xu L, Cheng YS, Chen CC, Guo RT. Structural insight into catalytic mechanism of PET hydrolase, Nat. Commun., vol. 8, no. 1, p. 2106, Dec. 2017, doi: 10.1038/s41467-017-02255-z.
3. Liu C, Shi C, Zhu S, Wei R, Yin CC. Structural and functional characterization of polyethylene terephthalate hydrolase from Ideonella sakaiensis, Biochem. Biophys. Res. Commun., vol. 508, no. 1, pp. 289–294, Jan. 2019, doi: 10.1016/j.bbrc.2018.11.148.
4. Shi L, Liu H, Gao S, Weng Y, Zhu L. Enhanced Extracellular Production of Is PETase in Escherichia coli via Engineering of the pelB Signal Peptide, J. Agric. Food Chem., vol. 69, no. 7, pp. 2245–2252, Feb. 2021, doi: 10.1021/acs.jafc.0c07469.
5. Son HF, Joo S, Seo H, Sagong HY, Lee SH, Hong H, Kim KJ. Structural bioinformatics-based protein engineering of thermo-stable PETase from Ideonella sakaiensis, Enzyme Microb. Technol., vol. 141, p. 109656, Nov. 2020, doi: 10.1016/j.enzmictec.2020.109656.
6. Yinglu C, Yanchun Ch, Xinyue L, Saijun D, Yu’ e T, Yuxin Q, Ruchira M, Jing H, Chunli L, Xu H, Weidong L, Quan Ch, Wangqing W, Xin W, Wenbin D, Shuangyan T, Hua X, Haiyan L, Yong L, Kendall N, Bian W. Computational Redesign of a PETase for Plastic Biodegradation under Ambient Condition by the GRAPE Strategy, ACS Catal., vol. 11, no. 3, pp. 1340–1350, Feb. 2021, doi: 10.1021/acscatal.0c05126.
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8. https://www.neb.com/en/protocols/0001/01/01/protocol-for-expression-using-neb-express-2523.
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