ANTIMIKROB VA FUNGITSID TA'SIRIGA EGA BO'LGAN O'SIMLIK DEFENSININING REKOMBINANT TURINI OLISH
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Plants have proteins and peptides that destroy microorganisms by affecting their membranes. These compounds are more effective than antibiotics and can be used to create pathogen-resistant transgenic organisms. However, the search and isolation of antimicrobial peptides from natural objects is a complex and lengthy process. As a result of our work, the activity of plant-derived peptides was studied, recombinant DNA with the Ns-D2 defensin gene was constructed, and the antimicrobial and fungicidal activity of recombinant defensin was confirmed. Thus, an alternative and more accessible system for obtaining it is proposed.
Владимир ЦОЙ, Дильбар ДАЛИМОВА, Шахноза ИБРАГИМОВА, Музаффар МУМИНОВ, Дарья ЗАКИРОВА, & Шахло ТУРДИКУЛОВА. (2024). ANTIMIKROB VA FUNGITSID TA’SIRIGA EGA BO’LGAN O’SIMLIK DEFENSININING REKOMBINANT TURINI OLISH. «O‘zMU XABARLARI», 3(3.1), 162–165. https://doi.org/10.69617/nuuz.v3i3.1.1752
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2. Broekaert WF, Terras FRG, Cammue BPA, Vanderleyden J. An automated quantitative assay for fungal growth inhibition. FEMS Microbiology Letters. 1990;69:55–9.
3. Mee Do H, Chul Lee S, Won Jung H, Hoon Sohn K, Kook Hwang B. Differential expression and in situ localization of a pepper defensin (CADEF1) gene in response to pathogen infection, abiotic elicitors and environmental stresses in Capsicum annuum. Plant Science. 2004;166:1297–305.
4. Huang HW. Action of antimicrobial peptides: two-state model. Biochemistry. 2000;39:8347–52.
5. Mosolov VV, Valueva TA. [Proteinase inhibitors and their function in plants: a review]. Prikl Biokhim Mikrobiol. 2005;41:261–82.
6. Ganz T. Defensins: antimicrobial peptides of innate immunity. Nat Rev Immunol. 2003;3:710–20.
7. Hannan MA, Rahman MA, Sohag AAM, Uddin MJ, Dash R, Sikder MH, et al. Black Cumin (Nigella sativa L.): A Comprehensive Review on Phytochemistry, Health Benefits, Molecular Pharmacology, and Safety. Nutrients. 2021;13:1784.
8. Rogozhin EA, Oshchepkova YI, Odintsova TI, Khadeeva NV, Veshkurova ON, Egorov TA, et al. Novel antifungal defensins from Nigella sativa L. seeds. Plant Physiol Biochem. 2011;49:131–7.
9. Rodrigues da Cunha L, Fortes Ferreira CLL, Durmaz E, Goh YJ, Sanozky-Dawes R, Klaenhammer T. Characterization of Lactobacillus gasseri isolates from a breast-fed infant. Gut Microbes. 2012;3:15–24.
10. Bulet P, Stöcklin R. Insect antimicrobial peptides: structures, properties and gene regulation. Protein Pept Lett. 2005;12:3–11.
11. Chen G-H, Hsu M-P, Tan C-H, Sung H-Y, Kuo CG, Fan M-J, et al. Cloning and characterization of a plant defensin VaD1 from azuki bean. J Agric Food Chem. 2005;53:982–8.
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