KINETICS OF NITROGEN CONSUMPTION BY THE MICROALGA DUNALIELLA SALINA IN BATCH CULTURE
The dynamics of biomass accumulation and mineral nitrogen uptake by Dunaliella salina under batch cultivation conditions were
investigated. The culture exhibited a typical sigmoid growth pattern, comprising the lag, exponential, stationary, and decline phases.
The maximum biomass concentration (1.10 g L⁻¹) was achieved on the fifth day of cultivation and was accompanied by intensive
nitrogen consumption. By the eighth day, nitrogen in the culture medium was almost completely depleted, resulting in growth
cessation and a subsequent decline in biomass to 0.30 g L⁻¹ by the end of the experiment. A pronounced inverse relationship
between nitrogen concentration and biomass accumulation was observed, confirming the limiting role of nitrogen in the growth of
D. salina. The results indicate that the optimal cultivation period for maximum biomass production is 5–7 days, whereas prolonged
cultivation under nitrogen-deficient conditions may be applied to induce the biosynthesis of β-carotene and neutral lipids. These
findings provide a scientific basis for optimizing technological regimes for the industrial cultivation of D. salina.
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