PHYTOCHEMICAL ANALYSIS AND UREASA INHIBITING ACTIVITY OF FUNGI ISOLATED FROM ALOE VERA
In this study we have researched urease inhibiting activity of 13 endophytic fungi derived from plant Aloe vera. Extracts of endophytic isolates AV5L (88,1%), AV6L (88,0%), AV4L (60%) and AV8R (63%) from plant Aloe vera demonstrated great inhibiting activity concerning urease. For comparison, leaf extract of Aloe vera revealed moderate inhibiting activity (50%), that concede to the major researched fungi. The standard urease inhibitor thiourea demonstrated the highest level of inhibition (94%).
The strains that demonstrated the highest inhibitory activity against urease were tested for phytochemical activity. Flavonoid, terpenoids, tannins, cardiac glycosides and peptides were revealed in those endophyte extracts. The presence of these classes of compounds indicates the potential of these strains as sources of biologically active substances responsible for the inhibition of urease. The results confirm the promising use of endophytic isolates of Aloe vera, which have significant potential as a source of natural urease inhibitors.
1. Anuradha Balasubramanian, Karthe Ponnuraj. Crystal structure of the first plant urease from jack bean: 83 years of journey from its first crystal to molecular structure.Mol.Biol.2010Jul16;400(3):274-83. doi:0.1016/j.jmb. 2010.05.009. Epub 2010 May 13.
2. A.E, Ajadi, A.K.Ajijolakewu, T.A.Sorunke, M.M.Suleiman, S.AAyoola and O.O Abdulai. Therapeutic Potential of Secondary Metabolities from Endophytic Fungi in Biotechnology and Medicine. Nig.J.Pure and Appl.Sci.Vol. 37 (Issue 1,2024) e-ISSN 2756-4045
3. Alvarez, M., et al. (2018). Peptides as Urease Inhibitors: Current Progress and Future Directions. Peptide Science, 108(4), 1-7. DOI: 10.1002/peps.23721
4. Dominika Matczuk, Anna Siczek. Effectiveness of the use of urease inhibitors in agriculture: a review/ International Agrophysics, 2021,35,197-208.
5. Iwona Koniecznal, Paulina Arnowiec, Marec Kwinkowski, Beata Kolesinska, Jastuna Fraczyk, Zbigniew Kaminski and Wieslaw Kaca. Bacterial Ureasa and its role in long lasting human diseases. Current protein and peptide science, 2012, vol13, No.8
6. J.P.Zhang, L.X.Chen, H. B.Lu,W.T. Lin "Urease Inhibitory Activity of Alkaloids from Fusarium and Aspergillus Endophytic Fungi" Phytochemistry.Volume:75,Issue:4,P.87-94,Year: 2017.
7. Ibrahim Kahramonoglu, Chuying Chen, Jinyin Chen and Chunpeng Wan. Chemical Constituents, Antimicrobial Activity, and Food Preservative Characteristics of Aloe vera Gel. Agronomy 2019, 9 (12), https://doi.org/10.3390/agronomy9120831
8. Rahul Yadav,Ajay Veer Singn, Samiksha Joshi and Manish Kumar. Antifungal and enzyme activity of endophytic fungi isolated from Ocimum and Aloe vera. Africa journal of Microbiology research. Vol.9(29), 2015.
9. Bhagobaty, R.K., & Joshi, S.R. (2011). Antimicrobial activity of endophytic fungi isolated from Centella asiatica and Aloe vera. International Journal of Pharmaceutical Sciences and Research, 2(3), 104–109.
10. Strobel G, Yang X, Sears J, Kramer R, Sidhu R.S., and Hess W. M., “Taxol from Pestalotiopsis microspora, an endophytic fungus of Taxus wallachiana,” Microbiology (Reading), vol. 142 (Pt 2), pp. 435–440, Feb. 1996, doi: 10.1099/13500872-142-2-435.
11. Wang, X., Min, C., Ge, M., & Zuo, R. (2013). An Endophytic Sanguinarine-Producing Fungus from Macleaya cordata, Fusarium proliferatum BLH51. Current Microbiology, 68(3), 336–341. https://doi.org/10.1007/s00284-013-0482-7.
12. Hazalin N.A., Ramasamy K., Lim S.M., Wahab I.A., Cole A.Lj, Majeed A.A.: Cytotoxic and antibacterial activities of endophytic fungi isolated from plants at the National Park, Pahang, Malaysia. BMC Complementary and alternative medicine. 2009, 9:46.
13. Prabhavathi RM, Prasad MP, Jayaramu M. Studies on Qualitative and Quantitative Phytochemical Analysis of Cissus quadrangularis. Adv Appl Sci Res 2016; 7:11-17.
14. Weatherburn M.W. (1967). Phenol-Hypochlorite Reaction for Determination of Ammonia. Analytical Chemistry, 39(8),971-974.
15. Seyedan, A., et al. (2019). Flavonoids as Natural Urease Inhibitors: A Review of Mechanisms and Therapeutic Potential. Journal of Enzyme Inhibition and Medicinal Chemistry, 34(1), 1-13. DOI: 10.1080/14756366.2019.1582493.
16. Shabnam Mahernia, Kowsar Bagherzadeh, Faraz Mojab and Massoud Amanlou. Ureasa Inhibitory Activities of some Commonly Consumed Herbal Medicines. Iranian Journal of Pharmaceutical Research, January 2014.
17. Shokrzadeh, M., et al. (2021). Tannins as Urease Inhibitors: A Review on Their Mechanisms and Therapeutic Potential. Phytotherapy Research, 35(2), 389-404. DOI: 10.1002/ptr.6785
18. Shokrzadeh, M., et al. (2021). Tannins as Urease Inhibitors: A Review on Their Mechanisms and Therapeutic Potential. Phytotherapy Research, 35(2), 389-404. DOI: 10.1002/ptr.6785
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