ECO-FRIENDLY ALTERNATIVE: NUTRIENT BROTH BASED ON APPLE AND POTATO WASTE FOR THE GROWTH OF BACILLUS SUBTILIS

agri-food waste, Bacillus subtilis, alternative medium, biotechnology, apple pomace, potato peel, OD₆₀₀, CFU/mL, biomass, eco-friendly medium

Authors

Recently, there has been growing interest in environmentally friendly methods in microbiology using agro-food waste. The aim of the study was to develop a nutrient medium based on apple-potato waste for cultivating Bacillus subtilis. Products from fruit and vegetable processing were used, which were treated thermally and enzymatically. The growth of the culture was evaluated using spectrophotometry, and the results were compared with the traditional LB medium