SYNTHESIS AND PHYSICO-CHEMICAL CHARACTERIZATION OF ECOLOGICAL CHITOSAN FROM APIS MELLIFERA HONEYBEE CHITIN
This article presents the results of synthesizing ecological chitosan from Apis Mellifera honeybee chitin by an improved alkaline
deacetylation method and its comprehensive physico-chemical characterization. Three synthesis methods were compared:
conventional alkali, microwave-assisted (1.5 h, 90% deacetylation degree), and ultrasound-assisted methods. FTIR spectroscopy
confirmed characteristic bands: N–H/O–H at 3350 cm⁻¹, C=O at 1655 cm⁻¹. TGA showed stability up to 200°C. The synthesized
chitosan (DD 85–90%, MW 150–250 kDa) shows high potential as an ecological additive for biodiesel fuel.
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