ISOLATION OF PYROLE-2-CARBOXYLIC ACID METHYL ESTER FROM A. BISPORUS MUSHROOM GROWING IN NATURAL CONDITIONS AND EXPLANATION OF ITS STRUCTURE USING SPECTROSCOPIC METHODS
A. bisporus mushroom is considered one of the leading mushrooms in the world in terms of consumption and chemical composition,
as well as its richness in various components. In the framework of this study, the substances obtained from A. bisporus mushroom
during the chloroform: methanol (5:1) system fractionation stage by column chromatography method were analyzed by GC-MS:
secondary metabolites belonging to the following classes were formed: complex esters, various heterocyclic compounds, organic
acids, etc. Among them, the substance pyrrole-2-carboxylic acid methyl ester was also found, and its chemical structure was
determined using physical research methods (IR, H-NMR, NMR).
1. Cao Jinfeng, Chen Xuhui, Liu Shiwei, Ding Jianhai. Research Advances in Secondary Metabolites and Biological Activities
of Mushroom Genus Fungi // Academic Journal of Agriculture & Life Sciences, 2023, 4(1); doi:
10.25236/AJALS.2023.040109.
2. Wen Li, Zhen Gu, Yan Yang, Shuai Zhou, Yanfang Liu, Jingsong Zhang. Non-volatile taste components of several cultivated
mushroomsm // Food Chemistry,2014, 143, 427-431, https://doi.org/10.1016/j.foodchem.2013.08.006
3. Shu-Yao Tsai, Hui-Li Tsai, Jeng-Leun Mau. Non-volatile taste components of Agaricus blazei, Agrocybe cylindracea and
Boletus edulis // Food Chemistry, 2008, 107(3), 977-983, https://doi.org/10.1016/j.foodchem.2007.07.080
4. Alexander J. MacLeod, Shnehlata D. Panchasara. Volatile aroma components, particularly glucosinolate products, of cooked
edible mushroom (Agaricus bisporus) and cooked dried mushroom // Phytochemistry,Volume 22, Issue 3,1983, Pages 705-
709
5. Cronin D. A., Margaret K. Ward. The characterisation of some mushroom volatiles // Food and Agriculture Science Journal,
Volume22, Issue9, 1971 477-479
6. Shao,Yang-yang and Gao,Hai-yan and Liu,Rui-ling and Fang,Xiang-jun and Chen,Hang-jun, ., Effect of harvesting method
on the quality and volatile flavor compounds of Agaricus bisporus // China Food Publishing, 2022, 43(5), 218–226.
7. Eugene Sebastian J. Nidiry. Structure–fungitoxicity relationships of some volatile flavour constituents of the edible
mushrooms Agaricus bisporus and Pleurotus florida. "Flavour and Fragrance Journal", 2001, 16(4), 245-248.
8. Bartkiene E, Zarovaite P, Starkute V, Mockus E, Zokaityte E, Zokaityte G, Rocha JM, Ruibys R, Klupsaite D. Changes in
Lacto-Fermented Agaricus bisporus (White and Brown Varieties) Mushroom Characteristics, including Biogenic Amine and
Volatile Compound Formation // Foods. 2023; 12(13):2441. https://doi.org/10.3390/foods12132441
9. Yan, S. J., Xie, X. M., Lin, S. Z., Xu, X. G., Jiang, S. P., Chen, B., ... & Liu, L. (2015). Study on volatile flavor components
from Agaricus bisporus of Tibet. Acta Scientiarum Naturalium Universitatis Sunyatseni, 54, 70-78.
10. Morosanova, M. A., Fedorova, T. V., Polyakova, A. S., & Morosanova, E. I. (2020). Agaricus bisporus crude extract:
Characterization and analytical application // Molecules, 25(24), 5996.
11. Consolacion Y Ragasa, Jo Madeleine Ann Reyes, Maria Carmen S Tan, Robert Brkljača, Sylvia Urban. 2016. Research
Article Sterols and Lipids from Agaricus bisporus // International Journal of Pharmaceutical and Clinical Research 2016;
8(10): 1451-1453.
12. Munkhgerel, L., Erdenechimeg, N., Dumaa, M., Zhang, G., Odonmajig, P., & Regdel, D. (2014). Chemical and biological
investigation of the Agaricus silvaticus Schaeff ex. Secr // Mongolian Journal of Chemistry, 12, 92–97.
https://doi.org/10.5564/mjc.v12i0.180
13. Shimizu T, Kawai J, Ouchi K, Kikuchi H, Osima Y, Hidemi R. Agarol, an ergosterol derivative from Agaricus blazei, induces
caspase-independent apoptosis in human cancer cells // Int J Oncol. 2016 Apr;48(4):1670-8. doi: 10.3892/ijo.2016.3391.
Epub 2016 Feb 15. PMID: 26893131.
14. Ueguchi Y, Matsunami K, Otsuka H, Kondo K. Constituents of cultivated Agaricus blazei // Journal of Natural Medicines,
2011 65(2):307-12. DOI:10.1007/s11418-010-0495-5
15. Petrova A, Alipieva K, Kostadinova E, Antonova D, Lacheva M, Gjosheva M, Popov S, Bankova V. GC-MS studies of the
chemical composition of two inedible mushrooms of the genus Agaricus // Chem Cent J. 2007 Dec 20;1(33). doi:
10.1186/1752-153X-1-33. PMID: 18096035; PMCID: PMC2228291
16. Nomozova, M.Z., Kamolov, L. S., Nakhatov I., Nuraliyeva D.I. Extraction of secondary metabolites from agaricus bisporus
fungus // Universum: химия и биология. 2026, fevral, 2(140), pp 26-30
Copyright (c) 2026 «ACTA NUUz»
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
.jpg)
1.png)
