MOLEKULYAR ABSORBSION USULLAR ORQALI GLYUKOKORTIKOIDLAR ASOSIDAGI KO‘P KOMPONENTLI DORI VOSITALARINI ANIQLASH USULLARINI TAKOMILLASHTIRISH
This article presents the determination of multi-component pharmaceutical preparations containing glucocorticoids using molecular absorption spectrophotometry. Hydrocortisone, prednisolone, and dexamethasone showed maximum absorption at 238–247 nm. The method demonstrated high linearity (R² > 0.997), low detection limits (0.07–0.09 μg/ml), and good repeatability (RSD < 2.5%). Due to its simplicity, cost-effectiveness, and applicability, the approach is suitable for pharmaceutical quality control.
1. Kudryashova I.V., Petrova A.N. Spectrophotometric determination of corticosteroids in pharmaceuticals. Journal of Analytical Chemistry, 2015; 70(6): 675–681.
2. Karimov B., Akhmedov A. Modern UV-spectroscopy methods for multi-component drug analysis. Uzbek Chemical Journal, 2020; (3): 45–51.
3. Sidorova T.Yu., Popov A.P. Advances in absorption spectroscopy for pharmaceutical control. Pharmaceutical Chemistry Journal, 2018; 52(4): 301–307.
4. Zhou M., Liu X. Development of sensitive spectrophotometric assays for steroid hormones. Analytical Sciences, 2019; 35(2): 155–162.
5. Aliev K.B., Rashidova Sh.S. Comparative study of glucocorticoid quantification methods. Khimiya i Tekhnologiya Lekarstvennykh Preparatov, 2021; (5): 32–38.
6. Ganiev F.N., Saidova D.K. Complexation-based UV methods for drug mixtures. Uzbek Journal of Chemistry, 2019; (2): 23–29.
7. Li Y., Wang J. Direct UV analysis of corticosteroids in combined dosage forms. International Journal of Pharmacy, 2018; 543(1): 102–108.
8. Rashidova Sh.S., Ziyayeva N.T. Spectrophotometric determination of prednisolone without derivatization. Chemistry of Natural Compounds, 2021; 57(3): 492–498.
9. Singh R., Verma A. Modified chromogenic reagents for selective steroid detection. Talanta, 2020; 214: 120866.
10. Garcia A., Ruiz B., Barbas C. HPLC determination of corticosteroids in pharmaceutical formulations using UV detection. Journal of Pharmaceutical and Biomedical Analysis, 2002; 29(1–2): 15–22.
11. Wang S., Xu L., Zhang C. Development and validation of an LC-MS/MS method for simultaneous quantification of multiple glucocorticoids in plasma. Analytical Methods, 2015; 7(6): 2340–2348.
12. Patel M., Shah D., Patel K. UV-spectrophotometric method for estimation of prednisolone in bulk and pharmaceutical dosage form. Int. J. Pharm. Sci. Rev. Res., 2010; 3(2): 83–86.
13. Rahmonova D.R., Karimova S.A., Umarova M.N. Application of molecular absorption spectrophotometry in the analysis of corticosteroid-containing medicines. Uzbek Journal of Pharmaceutical Sciences, 2021; 3(1): 45–51.
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