CHARACTERIZATION OF PROTEOLYTIC ACTIVITY OF SOIL MICROFLORA THROUGHOUT THE ARAL
The use of chemicals in various fields around the world has increased to the extent that it affects people’s health. The modern
world intends to replace these harmful chemicals with environmentally friendly products to improve life on our planet. Despite
the chemical processes, the establishment of enzymatic processes has been the main goal of scientists. Various enzymes,
particularly microbial proteases, are most widely used in various corporate sectors such as textiles, detergents, leather, feed,
waste, etc. For this purpose, 16 soil samples were collected from the Aral Sea. 306 bacterial isolates were randomly selected from
16 soil samples based on their color. It is determined that 51 bacterial isolates showed protease activity using casein nutrient
medium.
1. Amoozegar, M. A., Fatemi, A. Z., Karbalaei-Heidari, H. R., and Razavi, M. R. (2007). Production of an extracellular
alkaline metalloprotease from a newly isolated, moderately halophile, Salinivibrio sp. strain AF-2004. Microbiol. Res. 162,
369–377. doi: 10.1016/j.micres.2006.02.007
2. Barrett, A. J., and McDonald, J. K. (1986). Nomenclature: protease, proteinase and peptidase. Biochem. J. 237:935. doi:
10.1042/bj2370935
3. Beg, Q. K., and Gupta, R. (2003). Purification and characterization of an oxidation-stable, thiol-dependent serine alkaline
protease from Bacillus mojavensis. Enzyme Microb. Technol. 32, 294–304. doi: 10.1016/S0141-0229(02)00293-4
4. Di Salvo JW (1958) Desoxyribonuclease and coagulase activity of micrococci. Med Tech Bull 9:191-196
5. Garriga M, Hugas M, Gou P, Aymerich MT, Arnau J, Monfort JM (1996) Technological and sensorial evaluation of
Lactobacillus strains as starter cultures in fermented sausages. Int J Food Microbiol 32(1-2):173-183
6. Gessesse, A. (1997). The use of nug meal as a low-cost substrate for the production of alkaline protease by the
alkaliphilic Bacillus sp. AR-009 and some properties of the enzyme. Bioresour. Technol. 62, 59–61. doi:10.1016/S0960-
8524(97)00059-X
7. Gupta, A., Joseph, B., Mani, A., and Thomas, G. (2008). Biosynthesis and properties of an extracellular thermostable serine
alkaline protease from Virgibacillus pantothenticus. World J. Microbiol. Biotechnol. 24, 237–243. doi: 10.1007/s11274-
007-9462-z
8. Kang, S. G., Kim, I. S., Rho, Y. T., and Lee, K. J. (1995). Production dynamics of extracellular proteases accompanying
morphological differentiation of Streptomyces albidoflavus SMF301. Microbiology 141, 3095–3103. doi:
10.1099/13500872-141-12-3095
9. Kasana RC, Salwan R, Yadav SK (2011) Microbial proteases: detection, production, and genetic improvement. Crit Rev
Microbiol 37(3):262-276
10. Kumar, C. G., and Takagi, H. (1999). Microbial alkaline proteases: from a bioindustrial viewpoint. Biotechnol. Adv. 17,
561–594. doi: 10.1016/S0734-9750(99)00027-0
11. Miyaji, T., Otta, Y., Nakagawa, T., Watanabe, T., Niimura, Y., and Tomizuka, N. (2006). Purification and molecular
characterization of subtilisin-like alkaline protease BPP-A from Bacillus pumilus strain MS-1. Lett. Appl. Microbiol. 42,
242–247. doi: 10.1111/j.1472-765X.2005.01851.x
12. Nisha, N., and Divakaran, J. (2014). Optimization of alkaline protease production from Bacillus subtilis NS isolated from
sea water. Afr. J. Biotechnol. 13, 1707–1713. doi: 10.5897/AJB2014.13652
13. Oberoi, R., Beg, Q. K., Puri, S., Saxena, R., and Gupta, R. (2001). Characterization and wash performance analysis of an
SDS-stable alkaline protease from a Bacillus sp. World J. Microbiol. Biotechnol. 17, 493–497. doi:
10.1023/A:1011918806201
14. Palsaniya, P., Mishra, R., Beejawat, N., Sethi, S., and Gupta, B. L. (2012). Optimization of alkaline protease production
from bacteria isolated from soil. J. Microbiol. Biotechnol. Res. 2, 695–701.
15. Pastor, M. D., Lorda, G. S., and Balatti, A. (2001). Protease obtention using Bacillus subtilis 3411 and amaranth seed meal
medium at different aeration rates. Braz. J. Microbiol. 32, 6–9. doi: 10.1590/S1517-83822001000100002
16. Rao, M. B., Tanksale, A. M., Ghatge, M. S., and Deshpande, V. V. (1998). Molecular and biotechnological aspects of
microbial proteases. Microbiol. Mol. Biol. Rev. 62, 597–635
17. Rao, M. B., Tanksale, A. M., Ghatge, M. S., and Deshpande, V. V. (1998). Molecular and biotechnological aspects of
microbial proteases. Microbiol. Mol. Biol. Rev. 62, 597–635.
18. Rifaat, H. M., El-Said, O. H., Hassanein, S. M., and Selim, M. S. (2007). Protease activity of some mesophilic
streptomycetes isolated from Egyptian habitats. J. Cult. Collect. 5, 16–24.
19. Simkhada, J. R., Cho, S. S., Park, S. J., Mander, P., Choi, Y. H., Lee, H. J., et al. (2010a). An oxidant-and organic solvent-
resistant alkaline metalloprotease from Streptomyces olivochromogenes. Appl. Biochem. Biotechnol. 162, 1457–1470. doi:
10.1007/s12010-010-8925-0
20. Zerdani I, Faid M, Malki A (2004) Feather digestion by new isolated strains Bacillus sp. in microcco. Afr J Biotechnol 3:
67- 70.
Copyright (c) 2026 «ACTA NUUz»
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
.jpg)
1.png)
